We seed a suitable growth medium with an appropriate inoculum of cells known to be capable of replicating in that growth medium. ![]() ![]() “Bacterial populations in both batch and continuous culture are much more heterogeneous than is normally assumed, and such cultures may consist of several types of subpopulations simultaneously differing in viability, activity and integrity of the cells.”Ĭonsider a typical axenic flask or broth culture of bacteria (įigure 1), arguably the staple of modern laboratory microbiology. “It is now well established that some micro-organisms can, under certain conditions, be deprived of all visible signs of life and yet these organisms are not dead, for, when their original conditions are restored, they can return to normal life and activity.”
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